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. 2016 Jan 20;6:19197. doi: 10.1038/srep19197

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(a) Expression of selected VCS-enriched genes, shown as the log2 expression ratio between Purkinje fibers (PF) and working myocardium (WM). Gene are color-coded for Nkx2-5-Irx-VCS (green), Tbx5-Irx-VCS (red), and Nkx2.5-Tbx5-Irx-VCS (yellow). (b) qPCR experiment demonstrates that Irx3 activates most of VCS-enriched genes in cardiomyocytes, shown as the log2 expression ratio between Ad-Irx3 and Ad-GFP. n = 3–5 for each group. *P < 0.05 vs. Ad-GFP.