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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Curr Protoc Hum Genet. 2016 Jan 1;88:21.4.1–21.4.23. doi: 10.1002/0471142905.hg2104s88

Figure 3. HDR Donor Plasmid Design and Assembly Workflow.

Figure 3

(A) Example genomic region of interest for targeted transgene recombination. (B) Theoretical recombinant genomic region of interest with 3× nuclear localized fluorescent reporter and 2A peptide sequences. (C) Diagram of 3 insert cloning with Cold Fusion/IN-Fusion with restriction enzyme digested pENTR1A vector. (D) Diagram of complete donor plasmid construct. See Li and Elledge, 2007, for the principles behind Cold Fusion/IN-Fusion technologies.