Table 2. Materials Used in This Unit.

MATERIAL	Provider	Catalog #	Amount
hES Cell Media (Option 1) Store for 1 week at 4°C			500mL
MTesR1	StemCell Technologies	05850
hES Cell Media (Option 2) Store for 1 week at 4°C			500mL
Essential 8 Medium	Life Technologies	A1517001
Cryopreservation Solution			100mL
0.22uM Filtered FBS	any	any	90mL
Dimethyl Sulfoxide (DMSO)	any	any	10mL
Matrigel Substrate Store for 1 week at 4°C
Corning® Matrigel® hESC-Qualified Matrix *See lot-by-lot dilution instructions, generally ∼100×;Thaw on ice at 4°C for 4 hours or overnight to reduce precipitation.	Corning (BD)	354277	Variable*
Knockout DMEM	Life Technologies	10829-018	Variable*
Lysis Buffer			As Needed
Tris pH7.5	any	any	[10 mM]
EDTA pH 8.0	any	any	[10 mM]
NaCl	any	any	[10 mM]
N-Lauroylsarcosine	any	any	[0.5% vol/vol]
Proteinase K - Add immediately before use.	any	any	[1 mg/ml]