Table 1. Primers used for amplification, sequencing and expression analysis.
| Primer name | Sequence (5'→3') | ORFsa | Amplicon size | Reference |
|---|---|---|---|---|
| NGT1-F | TACGGCATTGGAACAACCC | 19.5392 | 1804 bp | This study |
| NGT1-R | CCAACAGGATAGATTAGCCAACC | 19.5392 | -- | -- |
| NGT1-2Fb | ATGATTTGTGGATCAGTATTGGC | 19.5392 | 968 bp | This study |
| NGT1-2Rc | CGTCTTTCCAGTAAGGGTGAGT | 19.5392 | 942 bp | This study |
| NAG1-F | GCATTCGGTTGTTCTGGGAG | 19.2156 | 1098 bp | This study |
| NAG1-R | CCTTCAACTCTTATCTGTCGCC | 19.2156 | -- | -- |
| HXK1-Fa | CGCCAGTTGAATAAGATGGC | 19.2154 | 1178 bp | This study |
| HXK1-Ra | GGCGACAGATAAGAGTTGAAGG | 19.2154 | -- | -- |
| HXK1-Fbd | CGGTGTATTCTGCTGGGTG | 19.2154 | 898 bp | This study |
| HXK1-Rbe | CACCCAGCAGAATACACCG | 19.2154 | 896 bp | This study |
| DAC1-Fa | CTCCCAGAACAACCGAATGC | 19.2157 | 1459 bp | This study |
| DAC1-Ra | CGACGAATGGGCAACCTC | 19.2157 | -- | -- |
| DAC1-Fbf | TGCCACCCAGTCGAAACG | 19.2157 | 930 bp | This study |
| NGT1-RT-Rg | TGGACCGATATAAGCACCATC | 19.5392 | 491 bp | This study |
| NAG1-RT-F | GCATTGAGTGTTGGTATTTCCACC | 19.2156 | 195 bp | This study |
| NAG1-RT-R | ACTTTAATCCAGCGGCAGC | 19.2156 | -- | -- |
| HXK1-RT-F | CAAATTGTTGGGCAGTGACG | 19.2154 | 197 bp | This study |
| HXK1-RT-R | TGATAGGCAGCACCTATGGC | 19.2154 | -- | -- |
| DAC1-RT-F | CGTAACCTAGTCAAGTGGTCGC | 19.2157 | 198 bp | This study |
| DAC1-RT-R | TATCTGACGACTGGACTTCACG | 19.2157 | -- | -- |
| ACT1-RT-F | TCCAGAAGCTTTGTTCAGACCAGC | 19.5007 | 170 bp | [24] |
| ACT1-RT-R | TGCATACGTTCAGCAATACCTGGG | 19.5007 | -- | -- |
aOpen reading frames (ORFs) were downloaded from www.candidagenome.org and used for primer design
bThis primer was used in combination with the reverse primer NGT1-R
cThis primer was used in combination with the forward primer NGT1-F
dThis primer was used in combination with the reverse primer HXK1-Ra
eThis primer was used in combination with the forward primer HXK1-Fa
fThis primer was used in combination with the reverse primer DAC1-Ra
gThis primer was used in combination with the forward primer NGT1-2F.
Primers used for expression analysis are labeled with "RT".