Fig 1. Association of Dt1 repression with Dt2 expression revealed by qRT-PCR.
(A) Expression levels of Dt1 and Dt2/dt2 in the stem tips of IA3023 and NE3001 at the V2 stage. Expression levels of dt2 in IA3023 and Dt1 in NE3001 were set as 1, and those of Dt2 in NE3001 and Dt1 in IA3023 were adjusted accordingly. (B) Expression levels of Dt1 and Pro35S:Dt2/dt2 in the stem tips of Thorne and Thorne transgenic line at the V2 stage. Expression levels of dt2 in Thorne and Dt1 in Thorne transgenic line were set as 1, and those of Pro35S:Dt2 in the transgenic line and Dt1 in Throne were adjusted accordingly. (C) Expression levels of Dt1 and Pro35S:Dt2/dt2 in non-transgenic and transgenic hairy roots of Kefeng 1. Expression levels of dt2 in non-transgenic hairy roots and Dt1 in transgenic hairy roots were set as 1, and those of Pro35S:Dt2 in transgenic roots and Dt1 in non-transgenic roots were adjusted accordingly. Values in the (A) and (C) are shown as mean δ standard errors of the means from three biological replicates, while values in the (B) are shown as mean δ standard errors of the means from three technical replicates.