Fig 2. Ultrametric phylogenetic tree demonstrating estimated speciation times of parental species and the Hybrid clade I.

Species-specific karyotypes arranged from Giemsa stained chromosomes are shown along the right side of cladogram. Confidence intervals of nodes of interest are in grey colour. TT, C. taenia; NN, C. tanaitica; EE, Cobitis elongatoides. Chromosomes were arranged in a decreasing size order and classified in four morphological groups: metacentric (m), submetacentric (sm), subtelocentric (st) and acrocentric (a).