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. 2016 Jan 25;11(1):e0146872. doi: 10.1371/journal.pone.0146872

Table 2. Individuals used in this study.

Biotype Country Locality Lat Long AOC NOI NOMKA NOGE NOAGM
EE Hungary Szodrakosz Cr. 47°43'58.8"N 19°07'58.8"E - 1 5 - -
Poland Budkowiczanka R. 50°50'50.1"N 18°11'07.1"E - 1* - - -
TT Germany Haaren Cr. 53°04'58.8"N 7°49'58.8"E - 2* + 1 4 - -
NN Romania Danube R. 44°04'47.9"N 26°43'51.2"E - 2* - - -
Romania Sinoe at Histria 44°37'58.8"N 28°52'58.8"E - 1 4 - -
ET Czech Rep. Laboratory F1 50°24'37.6"N 14°27'16.9"E F1 generation 1 9 1 4
Poland Dolna Barycz R. 51°36'59.1"N 16°30'49.1"E Holocene 4 27 2 4
total 5 36 3 8
EN Bulgaria Jantra R. 43°09'58.0"N 25°55'53.8"E hybrid clade I 2 22 1 1
Romania Danube R. 44°04'47.9"N 26°43'51.2"E hybrid clade I 3 15 2 9
total 5 37 3 10
EET Germany Neisse R. 51°51'28.8"N 14°36'34.9"E Holocene 1 12 1 8
Poland Polska Woda R. 51°31'17.0"N 17°30'07.0"E Holocene 3 16 - -
Poland Dolna Barycz R. 51°36'59.1"N 16°30'49.1"E Holocene 2 5 1 2
Czech Rep. Pšovka Cr. 50°22'11.8"N 14°33'06.8"E Holocene 1 2 - -
total 7 35 2 10
ETT Germany Issel R. 51°51'00.0"N 06°15'00.0"E Holocene 2 13 2 3
Germany Ilmenau R. 53°22'34.0"N 10°14'36.5"E Holocene 1 2 - -
Czech Rep. Laboratory B1 50°24'37.6"N 14°27'16.9"E B1 generation 3 17 2 10
total 6 32 4 13
EEN Bulgaria Vit R. 43°15'47.0"N 24°19'30.1"E hybrid clade I 2 56 2 6
Poland Polska Woda R. 51°31'17.0"N 17°30'07.0"E hybrid clade I 1 7 1 1
Romania Barlat R. 46°40'23.8"N 27°40'07.5"E Holocene 3 6 - -
Romania Comana R. 44°10'07.1"N 26°08'54.9"E hybrid clade I 1 2 - -
Romania Adjud R. 46°04'21.0"N 27°12'32.2"E Holocene 1 2 - -
Slovakia Cierna voda R. 48°36'27.0"N 21°59'34.1"E hybrid clade I 8 51 5 17
total 16 124 8 24
ENN Romania Danube R. 44°04'47.9"N 26°43'51.2"E Holocene 10 35 3 12
total 10 35 3 12

Abbreviations: Capital letters represent sets of haploid genomes: E, Cobitis elongatoides; T, C. taenia; N, C. tanaitica. Shortcuts in the column captions: AOC, age of clones; NOI, number of individuals; NOMKA, number of metaphases karyologically analysed; NOGE, number of GISH experiments; NOAGM, number of analysed GISH metaphases.

In order to prepare probes for GISH experiments only gDNA was used from individuals marked with *.