Fig 7. Co-immunoprecipitation of the Cnot7 mutants confirming the specific disruption of interaction with TOB1 by the E247A/Y260A mutant (A).

Co-immunoprecipitation of the Cnot7 mutants confirming the specific disruption of interaction with CNOT1 by the M141R mutant (B). Western blot confirming the equal expression of Cnot7 constructs in cells used for spontaneous metastasis assays (C). Dot plot of the primary tumor mass for Cnot7 wildtype and mutant cells orthotopically implanted into mice (D). Surface metastasis plot results of orthotopically implanted 4T1 cells expressing mutant Cnot7 constructs (E). Dot plot of the surface pulmonary metastasis count for orthotopically constructs normalized for primary tumor burden (F).