Fig 2. Hyperoxia-induced morphological alteration of alveolar macrophages is inhibited in ASK1 KO mice.

Bronchoalveolar lavage (BAL) fluid was collected from WT and ASK1 KO mice (n = 4 per each group) exposed to room air or 100% O₂ for 24 h (A-C). Photomicrographs show representative images of Diff-Quik stained BAL fluid cells. All cells shown here are mononuclear and have a relatively large cytoplasmic space, suggesting that these cells are macrophages or macrophage-committed mononuclear cells that were recruited to the lung airspace. Arrows denote macrophages with shrinking of cytoplasm. Magnification (A, B, & C): 400X.