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. 2016 Jan 25;11(1):e0147962. doi: 10.1371/journal.pone.0147962

Fig 3. Electrophoretic analysis of RT-LAMP amplification products with WEEV nsP4 and SLEV 3’ UTR primer sets.

Fig 3

The gel is a 2% agarose E-gel (Invitrogen) with ethidium bromide. The marker (“M”) is a Low Molecular Weight DNA Ladder (New England Biolabs). Lane 1: WEEV positive control reaction with 2 PFU equivalent WEEV RNA in 20 μL. Lane 2: WEEV positive control after digestion with XbaI. Lane 3: No-template control reaction with WEEV nsP4 primer set. Lane 4: no sample. Lane 5: SLEV positive control reaction with 2 PFU equivalent SLEV RNA in 20 μL. Lane 6: SLEV positive control after digestion with HindIII. Lane 7: No-template control reaction with SLEV 3’-UTR primer set. Cutting sites within the amplicon regions are shown in Figs 1 and 2.