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. 2016 Jan 25;11(1):e0147962. doi: 10.1371/journal.pone.0147962

Fig 5. Melt derivative curves for singleplex and multiplex RT-LAMP.

Fig 5

Solid black lines show average data from triplicate runs. Colored shaded areas represent the contributions of individual RT-LAMP targets to the overall signals to aid visualization. Blue = WEEV. Yellow = SLEV. Red = WNV. A) Singleplex reactions. B) Three-way combination. Only two peaks are distinguishable by eye. C) WEEV + WNV no template control. A relatively small melting peak is present, corresponding to spurious amplification after 40 min. D-F) Pairwise combinations. A smaller WEEV peak is distinguishable from WNV and SLEV peaks in D and F. WNV and SLEV peaks are indistinguishable by eye in E.