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. 2016 Jan 25;10(1):e0004373. doi: 10.1371/journal.pntd.0004373

Fig 6. L-arginine deprivation induces externalization of phosphatidyl serine in Leishmania parasite.

Fig 6

Leishmania parasites were grown in L-arginine depleted (AD-Ld), L-arginine depleted but supplemented with ornithine (AD-Ld/Orn+), putrescine (AD-Ld/Put+), NAC (AD-Ld/NAC+) and L-arginine supplemented (AS-Ld) RPMI media for 72–120 hr. The cells were co-stained with Annexin V-FITC and PI followed by analysis by flow cytometry as described in “Materials and methods”. Histogram of the Annexin-V assay in different conditions as mentioned were shown in figures. The graphs were plotted and percent early apoptotic Leishmanial cells were represented. The data represents mean±SD of triplicate determinations and are representative of three independent experiments.*, P<0.05 (Student’s t test)**, P<0.001 compared to AD-Ld or AS-Ld parasite as applicable.