Fig 3. Zfrp8 affects cytoplasmic localization of select transcripts.
(A-C') Levels and localization of TAHRE transcripts (FISH, red) were low in control ovaries (A-A"), and strongly increased in the cytoplasm of Armi1/Armi72.1 ovaries (B-B', arrow). In Zfrp8 KD ovaries TAHRE RNA was present at similar levels in both nuclei and cytoplasm (D-D', arrows). (D-I') FISH with Pino (D-E), sta (F-G') and RpL36 (H-I") probes. Levels of Pino RNA is somewhat increased in Zfrp8 KD ovaries (E-E'), and significantly elevated in nurse cell nuclei (arrows in D' and E'). (F-G') sta transcripts did not show changes in levels or localization in Zfrp8 KD. (H-I') RpL36 transcript showed increased nuclear accumulation in Zfrp8 KD ovaries (arrows, in H' and I'). Additional images of ovarioles including germaria are shown in S4 Fig. DNA blue (DAPI), size bar is 20μm.