Fig 10. Effects of STO-609 on the CaMKI phosphorylation in chicken sperm.

Sperm incubated at 35°C in the –5mM Ca²⁺ or +5mM Ca²⁺ medium with or without CaMKKs inhibitor STO-609 (0; 1; 10; 50 μM) at 5 min and 15 min. The control (Ctrl): sperm incubated with or without Ca²⁺ in the absence of STO-609. Proteins from sperm lysates were analyzed by western-blotting using anti-phospho-Thr177-CaMKI or anti-CaMKI as the primary antibody. Bands for phospho-Thr177-CaMKI were detected at 41kDa (top lanes). Total CaMKI (41kDa) was used as loading control (bottom lanes) and the phosphorylated protein CaMKI (Thr177)/total CaMKI ratio is shown at the bottom. Results express the mean ± SEM of the mean from 4 different experiments. Different superscripts indicate statistically significant differences (P<0.05).