Fig 6. Effect of high D-glucose and experimental diabetes on the adenosine receptors content.
A. The effect of D-glucose exposure (24 h) on the protein levels of adenosine receptor subtypes A1, A2A, A2B and A3 in HK2 cells was evaluated by western blot. Representative images of western blots of total protein extracts from cells exposed to 5 and 25 mM D-glucose are shown. B. The effect of STZ-induced diabetes on adenosine A3 receptor content was evaluated by western blot of total protein extracts from tubules isolated from control (ctr) and diabetic (db) rats following 1 or 4 months post-induction. The graphs show quantitative analysis of immune signals. The means in controls were normalized to 1. A: n = 6, B: n = 3.