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. 2016 Jan 25;12(1):e1005813. doi: 10.1371/journal.pgen.1005813

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© 2016 Ku et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — The testes were stained with anti-Piwi (green), anti-VASA (germ cell, red), and anti-Tj (somatic cells, purple) antibodies. DNA was labeled by DAPI (blue). (A-A‴) Germline knockdown of Piwi using nosVP16-Gal4 driver. (B-B‴) The tsn¹¹²⁰/tsn⁰⁶¹⁴ mutant displayed a swollen apical tip of the testis. (C-C‴) The depletion of Piwi in germ cells rescued tsn mutant phenotype, suggesting TSN antagonizes the germline function of Piwi in regulating spermatogenesis.