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. 2016 Jan 25;11(1):e0147486. doi: 10.1371/journal.pone.0147486

Fig 5. HPLC analysis of BOA detoxification into HPMA by deletion mutants.

Fig 5

Chromatograms of (A) BOA and (B) HPMA standards (retention times of 10.9 and 6.69 min, respectively) in comparison to PDB amended with BOA that was either (C) left uninoculated (PDB+BOA) or was inoculated with (D) wild-type FRC M-3125 or the deletion mutants of (E) FVEG_08289 (AMD1), (F) FVEG_08290 (DLH1), or (G-H) FVEG_08291 (MBL1; two different deletion mutants). Wild type, Δamd1, and Δdlh1 were all able to fully metabolize BOA to HPMA. Two Δmbl1 mutants, Δ08291–33 and Δ08291–36, were unable to metabolize BOA. (I) Add-back of MBL1 (Δ08291–36::C2) complemented the deletion.