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. 2016 Jan 25;11(1):e0147693. doi: 10.1371/journal.pone.0147693

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© 2016 Zhao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Infiltration of monocytes/macrophages. ED-1-positive cells were detected by immunohistochemistry and calculated per high-power field (400 ×). (B) Immunohistochemistry and semi-quantitative analysis for MCP-1, TNFα, and IL-1β. (C) Real-time PCR for IL-1β, MCP-1, and TNFα. Original magnification, × 200. TSF-treated rats exhibit a significant inhibition of renal inflammation including ED-1, MCP-1, IL-1β, and TNFα. Data are expressed as means ± SE for each group of 9 rats. *P<0.05, **P<0.01 TSF-treated group vs. DN group; ^#P<0.05, ^##P<0.01, ^###P<0.001 DN group vs. normal group.