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. 2016 Jan 25;11(1):e0147693. doi: 10.1371/journal.pone.0147693

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© 2016 Zhao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) Immunohistochemistry (IHC) and quantitative analysis of collagen IV (Col-IV, original magnification, × 400), collagen I (Col-I, original magnification, × 200), and fibronectin (Fn, original magnification, × 200). (B) Western blot and quantitative analysis of collagen I, collagen IV, and fibronectin. (C) Real-time PCR of collagen I, collagen IV, and fibronectin. Results show that compared with the normal rats, renal fibrosisis markedly enhanced in DN rats. However, in TSF-treated DN rats renal fibrosis is inhibited as demonstrated by Western blot analysis, IHC at the protein level and real-time PCR at the mRNA level. Data are expressed as means ± SE for each group of 9 rats. *P<0.05, **P<0.01 TSF-treated group vs. DN group; ^#P< 0.05, ^##P<0.01, ^###P<0.001 DN group vs normal group.