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. 2016 Jan 25;11(1):e0147693. doi: 10.1371/journal.pone.0147693

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© 2016 Zhao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Immunohistochemistry. Original magnification,× 200and real-time PCR for TGF-β1. (B) Western blot and semi-quantitative analysis of renal TGF-β receptor II (TβRII), phosphorylation of TGF-β receptor I (p-TβRI) and TβRI, respectively. A marked activation of TGF-β/TGF-β receptor signaling in the diabetic kidney is blocked by TSF treatment. Data are expressed as means ± SE for each group of 9 rats. *P<0.05, **P<0.01 TSF-treated group vs. DN group; ^#P<0.05, ^##P<0.01, ^###P<0.001 DN group vs. normal group.