Fig. 1.

Exemplary gating strategy included in the panel’s guideline. The figure shows the gating strategy proposed in the guidelines and made of three sequential steps, allowing the identification of the 10 putative MDSC subsets. Step 1 consists in doublets exclusion followed by dead-cell exclusion. Step 2 allows the identification of three MDSC subsets (MDSC1-2-8), and from a consecutive gate, the other seven defined MDSC populations are identified in step 3