Table 3. Primers used to construct Caribbean chikungunya virus infectious clone.
Primer Name | Sequence (5’—-3’) |
---|---|
BamHI Forward | GATTAATAACCCATCATGGATCCTG |
AgeI Reverse | GTTGTAAATGGCCTGGACCGGTGTC |
AgeI Forward | GGTATATATTCTCGTCGGACACCGG |
AgeI2 Reverse | GGCTTCTTTTTCTTTTGAACCGGTT |
AgeI2 Forward | GCCCCCCAAAAAGAAACCGGTT |
XhoI Reverse | GTTACCCCACGTGACCTCGAGCC |
XhoI Forward | GTTAACCGTGCCGACTGAGGGGCTCG |
PolyANotI Reverse | GAGGATGCATTGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAATATTAAAAACAAAATAAC |
BamHI removal Forward | CCCATCATGGATtCTGTGTACGTGGATATAG |
BamHI removal Reverse | CTATATCCACGTACACAGaATCCATGATGGG |