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. 2016 Jan 25;10(1):e0004402. doi: 10.1371/journal.pntd.0004402

Table 3. Primers used to construct Caribbean chikungunya virus infectious clone.

Primer Name Sequence (5’—-3’)
BamHI Forward GATTAATAACCCATCATGGATCCTG
AgeI Reverse GTTGTAAATGGCCTGGACCGGTGTC
AgeI Forward GGTATATATTCTCGTCGGACACCGG
AgeI2 Reverse GGCTTCTTTTTCTTTTGAACCGGTT
AgeI2 Forward GCCCCCCAAAAAGAAACCGGTT
XhoI Reverse GTTACCCCACGTGACCTCGAGCC
XhoI Forward GTTAACCGTGCCGACTGAGGGGCTCG
PolyANotI Reverse GAGGATGCATTGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAATATTAAAAACAAAATAAC
BamHI removal Forward CCCATCATGGATtCTGTGTACGTGGATATAG
BamHI removal Reverse CTATATCCACGTACACAGaATCCATGATGGG