Table 5. Frequency of significant mutations (>0.001) identified in tissue culture passaged viral populations.

GenomeRegion	nt Position	nt Change	AA Change	C6/36-1	C6/36-2	C6/36-3	C6/36-4	C6/36-5	Vero-1	Vero-2	Vero-3	Vero-4	Vero-5
nsp1	479	T>C	syn>L	0.010		1.000		1.000
nsp1	1525	C>T	syn>Y	0.006				0.996		0.973		0.978	0.012
nsp2	2716	A>G	syn>T	0.996	0.999	0.997	0.997	0.997	0.999	0.999	0.999	0.999	0.999
nsp2	3059	G>A	G460S						0.371
nsp3	4507	C>A	syn>R	0.998	0.998	0.998	0.997	0.998
nsp3	4513	A>G	syn>K	0.999	0.999	0.999	0.999	0.996
nsp3	4864	T>A	syn>L	0.007	0.003	0.004	0.999	0.003	0.012	0.011	0.990	0.009	0.011
nsp4	7016	C>A	L455M			0.951	0.020	0.081
E2	8874	C>T	syn>F	0.005		0.002	0.002	0.002	0.990
E2	8892	T>C	syn>I	0.999		0.998		0.998	1.000	1.000	1.000	0.999	1.000
E1	10104	G>A	syn>T	0.979		0.002	0.003	0.004	0.002	0.002	0.003	0.002	0.968
E1	10802	G>T	G274V	0.044	0.041	0.096	0.093	0.088			0.959
3UTR	11775	C>T		0.978			0.952				0.985		0.981
3UTR	11776	G>A					0.999				0.995		0.994
3UTR	11791	C>T		0.359			0.291				0.499		0.539

nt = nucleotide, AA = amino acid, syn = synonymous, The table shows any mutation present above 0.1 in at least one individual, and any mutation above 0.001 found in more than one individual.