Table 1.

Real-time RT-PCR results and similarity searches of AFLP DD clones from poinsettia pedicel secondary abscission.

DD#	Primers used	GenBank Acc. #	Size (bp)	Real time RT-PCR resultsa	Discontinuous MegaBlast, BlastN, or BlastX
				Days after abscission induction
				0 1 2 3 4 5 6 7 Diste	Accession no.	Description	Species	Id (of 100%)	E-value
025a	29A	EB647682	194		GQ856147	Unknown mito. genome region	Citrullus lanatus	91b	9e-50
006a	33G	EB647681	269		XP_007012396	Photosystem II subunit X	Theobroma cacao	86d	3e-12
					AY340642	UVB-repressible protein	Trifolium pratense	79b	5e-31**
220_	29C	EB647701	515		XM_002528833	Transcription factor	Ricinus communis*	81b	5e-26
084_	40A	EB647705	456		AEJ07931	Opie3 pol protein	Zea mays	41d	4e-24
090a	26A	EB647690	255		XM_011094475	Organ-specific protein	Sesamum indicum	71c	0.020
					AY188755	Atypical receptor-like kinase	Zea mays	86c	8.2**
038b	62C	EB647683	233		CP011890	Unknown region of chr. 5	Ovis canadensis	86c	0.005
304b	PGTC	EB647707	217		HQ874649	Unknown mito. genome region	Ricinus communis*	96b	1e-61
057b	34A	EB647687	412		YP_002720125	Cytochrome f subunit (PetA)	Jatropha curcas*	96d	1e-65
208_	27A	EB647706	443		AY794600	Chloroplast tRNA-Leu	E. pulcherrima*	99b	3e-98
136b	GlcG	EB647697	447		XM_002512633	fk506-binding protein	Ricinus communis*	81b	3e-47
045c	1C	EB647684	347		XM_002510884	Uridylate kinase	Ricinus communis*	83b	2e-35
140b	PG0	EB647699	306		XM_002532624	eIF3E (translation initiation)	Ricinus communis*	84b	2e-28
103_	GlaG	EB647693	434		XM_012213718	CASP-like protein	Jatropha curcas*	77b	8e-36
003a	33A	EB647680	279		FJ228477	α-tubulin	Betula pendula	92b	2e-21
301_	PGA	EB647702	448		XM_002512412	RNA binding protein	Ricinus communis*	74b	1e-20
060c	34A	EB647688	304		XM_010526012	V-ATPase G subunit 1	Tarenaya hassleriana	83b	2e-09
320b	PGTG	EB647704	218		BT092277	unknown mRNA	Glycine max	87b	6e-07
082a	40G	EB647689	269		XM_002523025	Putative β-glucosidase	Ricinus communis*	72c	1e-06
101_	GlaG	EB647692	426		XM_002511291	Histone deacetylase	Ricinus communis*	71c	6e-06
140a	PG0	EB647698	307		AM932356	Partial tRNA-Leu gene	Typhonium giganteum	73c	1e-05
091b	26G	EB647691	247		CP001685	Glucan endo-1,3-β -D-glucosidase	Leptotrichia buccalis	90c	0.019
047b	6C	EB647685	236		XM_002305663	Proteasome beta subunit B family protein	Populus trichocarpa	82b	7e-08
130_	GlcG	EB647696	297		AY792209	NADH dehydrogenase SU 4L	Ceratitis neostictica	81c	3.5
304a	8C	EB647686	299		–	No significant matches	–	–	–
304a	GlaG	EB647694	343		–	No significant matches	–	–	–
304a	GlcG	EB647695	268		-	No significant matches	–	–	–
304a	GlcG	EB673117	249		-	No significant matches	–	–	–
204c	27G	EB647700	214		XM_012216503	Proteasome subunit alpha type-1-B-like	Jatropha curcas*	72c	1e-10
304a	PGA	EB647703	463		–	No significant matches	–	–	–

Similarities found in DNA sequences from $\mathit{chromosome}$, $\mathit{mitochondria}$, $\mathit{chloroplasts}$ or $\mathit{bacteria}$. List of primers in Supplement Materials.

^aVisualization of Real-time RT-PCR results relative to 18S expression of two individual experiments. The highest expression is black and the lowest expression is white, showing decreasing nuances of orange for descending steps of equal size (8 nuances/steps).

^bDiscontinuous MegaBlast [BlastN 2.2.32+, Database GenBank no (All GenBank + EMBL + DDBJ + PDB sequences) and standard settings 30 August 2015.

^cBlastN [BlastN 2.2.32+, Database GenBank no (All GenBank + EMBL + DDBJ + PDB sequences) and standard settings 30 August 2015.

^dBlastX Database no and standard settings 30. August 2015. Reading frame (RF) +1 of the Acc. # EB647681, +3 of the Acc. # EB647705 and EB647687.

^eInternal control: Distal part of the abscising pedicel above the abscission zone at Day 0.

^*Euphorbiaceae family member.

^**From BlastX in Sept 2012, incl because they give an additional indication of function.