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. 2016 Jan 25;11(1):e0147752. doi: 10.1371/journal.pone.0147752

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© 2016 Kossatz et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Specific uptake of PARPi-FL into FaDu (A) and Cal27 (B) cells was determined by flow cytometry after incubation of cells with PARPi-FL or Olaparib/PARPi-FL. Olaparib competes with PARPi-FL for specific binding sites on PARP1. PARPi-FL uptake of FaDu (C) and Cal27 cells (D) after irradiation with 2, 4 and 10 Gy was determined and compared to 0 Gy. Mean PARPi-FL fluorescence signal was measured via flow cytometry in the FITC channel. Bars represent means ± SEM from three independent experiments with three parallels each.