Fig 5. EM immunocolocalization of PLP-EGFP and HSV-1.

HOG-PLP cells were mock-infected or infected with HSV-1 at a m.o.i. of 100 and incubated for 1 h at 4°C. Then, cells were fixed and processed for observation by electron microscopy. Panels A, B and C show the presence of virions (arrows) attached to the plasma membrane localized next to colloidal gold particles (arrowheads) corresponding to PLP.