GluN2B mRNA expression levels decreased within the rACC following LV-GluN2B injection. Reverse transcription-quantiative polymerase chain reaction analysis of GluN2B mRNA expression levels in the (A) rACC, (B) vmPFC and (C) cACC following LV-GluN2B injection in the rACC (n=5–6 per group). No changes were detected in the mRNA expression levels of GluN2B in the cACC or vmPFC following LV-GluN2B injection. Data are presented as the mean ± standard error of the mean. *P<0.05 vs. the LV-NC group. GluN2B, GluN2B-containing NMDA receptors; rACC, rostral anterior cingulate cortex; cACC, caudal anterior cingulate cortex; vmPFC, ventromedial prefrontal cortex; NS, normal saline; LV-NC, lentiviral negative control; LV-GluN2B, lentiviral-mediated delivery of small interfering RNAs targeting GluN2B.