Attenuation of the protein expression levels of GluN2B in neurons of the rACC by intra-rACC administration of LV-GluN2B. (A) Western blot of GluN2B protein expression in the rACC neurons after intra-rACC delivery of NS, LV-NC or LV-GluN2B. (B) Western blot analysis demonstrated that GluN2B protein was significantly downregulated (49.6%) at day 7 following intra-rACC delivery of LV-GluN2B. *P<0.05 vs. the LV-NC or NS groups (n=4). GluN2B, GluN2B-containing N-methyl D-aspartate receptors; rACC, rostral anterior cingulate cortex; NS, normal saline; LV-NC, lentiviral negative control; LV-GluN2B, lentiviral-mediated delivery of small interfering RNAs targeting GluN2B.