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. 2015 Nov 11;11(1):33–42. doi: 10.3892/etm.2015.2854

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Comparison of the mean levels of (A and B) TLR4⁺ MNCs in the spleen, (C and D) CD4⁺CD25⁺ Treg cells and (E and F) CD4⁺CD25⁺Foxp3⁺ Treg cells in the peripheral blood at different temperatures and with different stimulants. Data (%) are presented as the mean ± standard deviation. (A and B) Analysis of the TLR4⁺ MNC data revealed the following: Comparison among multiple temperatures, F (2,108)=67.199, P<0.001; comparison among multiple stimulants, F (2,108)=2.129, P=0.126; LPS vs. Con-A, P=0.044. (C and D) Analysis of the CD4⁺CD25⁺ Treg cell data revealed the following: Comparison among multiple temperatures, F (2,108)=0.754, P=0.474; comparison among multiple stimulants, F (2,108)=0.532, P=0.589. (E and F) Analysis of the CD4⁺CD25⁺Foxp3⁺ Treg cell data revealed the following: Comparison among multiple temperatures, F (2,108)=7.984, P=0.001; comparison between temperatures and stimulants, F (4,108)=1.907, P=0.118; comparison between 20°C and −12°C, P<0.001; comparison between 4°C and −12°C, P=0.014. TLR, Toll-like receptor 4; CD, cluster of differentiation; Foxp3, Forkhead box P3; MNC, mononuclear cell; Treg, regulatory T; LPS, lipopolysaccharide; Con-A, concanavalin A.