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. 2015 Nov 11;11(1):33–42. doi: 10.3892/etm.2015.2854

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Comparison of the mean levels of (A) TLR4⁺ MNCs in the spleen, (B) CD4⁺CD25⁺ Treg cells and (C) CD4⁺CD25⁺Foxp3⁺ Treg cells in the peripheral blood at different times, temperatures and with different stimulants. Data (%) are presented as the mean ± standard deviation. (A) For the comparison of the TLR4⁺ data among multiple times: F (3,108)=64.432, P<0.001; 1 h vs. 12 h, P<0.001; 1 h vs. 24 h, P<0.001; 12 h vs. 48 h, P<0.001; 24 h vs. 48 h, P<0.001. For the comparison of the TLR4⁺ data between temperatures and times: F (6,108)=29.595, P<0.001. For the comparison of the TLR4⁺ data between times and stimulants: F (6,108)=2.281, P=0.045. For the comparison of the TLR4⁺ data among temperatures, times and stimulants: F (12,228)=1.954, P=0.042. (B) For the comparison of the CD4⁺CD25⁺ Treg data among multiple times: F (3,108)=1.568, P=0.205. (C) For the comparison of the CD4⁺CD25⁺Foxp3⁺ Treg cell data among multiple times: F (3,108)=13.322, P<0.001; 1 h vs. 48 h, P<0.001; 12 h vs. 48 h, P<0.001; 24 h vs. 48 h, P<0.001. For the comparison of the CD4⁺CD25⁺Foxp3⁺ Treg cell data between temperatures and times: F (6,108)=4.434, P=0.001. For the comparison of the CD4⁺CD25⁺Foxp3⁺ Treg cell data between times and stimulants: F (6,108)=2.397, P=0.036). TLR, Toll-like receptor 4; CD, cluster of differentiation; Foxp3, Forkhead box P3; MNC, mononuclear cell; Treg, regulatory T; LPS, lipopolysaccharide; Con-A, concanavalin A.