Table 1.

Brief comparison of transposase, recombinase, and integrase approaches

Approach	Advantage	Disadvantage
Transposases Sleeping beauty piggyBac	piggyBac	Reversible
Tyrosine recombinases Cre/loxP Flp/FRT	Less or no Amplification	Reversible
Serine integrases Φ C31	Irreversible integration in transcriptionally active the part of genome Stability Less or no amplification	Amplification is required for high-level expression