EIT-specific IgG and IgA. Test 1, intranasal immunization with electrosprayed nanoparticles chitosan-rEIT; Test 2, intranasal immunization with electrospraed TMC-rEIT nanoparticles; Control 1, intraperitoneally immunization with rEIT adjuvanted by Freund's adjuvant; Control 2, non-immunized mice nasally administered with chitosan alone; Control 3, non-immunized mice nasally administered with non-electrosprayed chitosan-rEIT. Mice immunization was conducted three times during 42 days. The sera, fecal, and eye wash samples were collected after each immunization and assayed for rEIT-specific responses, including (A) serum IgG titers, (B) serum IgA titers, (C) fecal IgA titers, (D) eye washes IgA titers using an ELISA method. Non-immunized mice and intraperitoneally immunized samples were used as negative and positive control, respectively (P<0.05).