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. 2015 Nov 23;11(1):610–618. doi: 10.3892/ol.2015.3957

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Melittin suppresses Cat S-induced invasion and angiogenesis of HUVECs. (A) Western blotting detected the expression of Cat S in transient transfection of HUVECs. β-actin served as a loading control. (B) Effect of melittin on invasion of transfected HUVECs was evaluated by Transwell chamber assay. Magnification, ×100. (C) Counted cell numbers from Transwell assay. Data are represented as the mean ± standard deviation of 3 independent experiments (*P<0.05 compared with control). (D) Effect of melittin on tube-formation in transfected HUVECs. Magnification, ×100. (E) Lengths of tubes were measured using Image-Pro Plus software. Data are expressed as the mean ± standard deviation. *P<0.05 compared with the control group. Cat S, cathepsin S; HUVECs, human umbilical vein endothelial cells.