Figure 1.

Expression of TRPM8 in tumor and non-tumor prostate cells. (A and B) Expression of TRPM8 in LNCaP, DU145 and PNT1A cells, detected by (A) RT-PCR and (B) western blot analysis. β-actin and GAPDH were used as positive controls in RT-PCR and western blot analysis, respectively. Water was used as a negative control in RT-PCR. (C and D) TRPM8 expression was quantified from (C) RT-PCR and (D) western blot analysis. LNCaP cells were used as positive controls for the expression of TRPM8. TRPM8, transient receptor potential melastatin 8; RT-PCR, reverse transcription-polymerase chain reaction.