Table II.
Immune phenotype markers.
| Cell types | Early separation | Reagents | Cell surface phenotype |
|---|---|---|---|
| DCs | Adherent cells (monocytes) | GM-CSF, IL-4, TNF, Aga | CD83+, CD86+, HLA2DR+ |
| CIK cells | Suspension cells (lymphocytes) | IFN2, CD3McAb, IL-2 | CD3+, CD8+, CD56+ |
a
DCs were presented with different tumor antigens that stimulate the immunoreactivity of DCs and promote cell proliferation, thereby enhancing the killing effect. DCs, dendritic cells; CIK, cytokine-induced killer; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; TNF, tumor necrosis factor; Ag, antigen; IFN, interferon; McAb, monoclonal antibody.