Figure 3.

Effects of sinomenine (SIN) on cyclooxygenase (COX)-2 protein and mRNA expression in SW1116 cells. SW1116 cells were treated with varying concentrations of SIN (0, 4, 8 and 10 mM) for 48 h (A and C). SW1116 cells were also treated with SIN (8 mM) for 24, 48 and 72 h. COX-2 protein and mRNA expression was examined by western blot analysis (A and B) and quantitative polymerase chain reaction analysis (C and D). The quantification of COX-2 protein and mRNA expression was normalized to GAPDH levels. Error bars represent means ± SEM. *P<0.05, **P<0.01, compared with corresponding control or 24 h group.