Fig. 5.

Proteome contents of lipoprotein fractions. Pooled plasma (0.25 ml; n = 3–5 per group) was separated by FPLC, as shown in Fig. 4, A–C. Fractions corresponding to VLDL (elution volumes: 14.0–16.0 ml), LDL (21.5–24.0 ml), and HDL (27.0–31.0 ml) were pooled for analysis. Lipid-bound proteins were isolated by LRA, trypsinized, and then analyzed by LC-MS/MS. For each sample, individual spectral counts for each protein were normalized to the total spectral count. The results are presented as the percent change in normalized spectrum counts for each identified protein in the mutant mice compared with the wild type for each lipoprotein class. HRG, histidine-rich glycoprotein.