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. 2015 Oct 29;11(1):405–410. doi: 10.3892/ol.2015.3842

Figure 4.

Figure 4.

miR-147 inhibited the activation of the Akt/mTOR signaling pathway. β-Actin was used as loading control. Ectopic expression of miR-147 downregulated Akt phosphorylation (p-Akt), P70S6K phosphorylation (p-P70S6K) and 4E-BP-1 phosphorylation (p-4E-BP-1), compared with blank control MDA-MB-231 cells (BC) and negative control miR-transfected cells (NC). Knockdown of Akt (si-Akt) reduced phosphorylation and the total level of Akt. The phosphorylation of P70S6K and 4E-BP-1 was also inhibited in the si-Akt group. The total P70S6K level was slightly upregulated in the si-Akt group.