Figure 3.
TGF-β and IL-10 in exosomes are responsible for immunosuppression. Concentrations of (A) TGF-β and (B) IL-10 in exosomes from MDA-MB-231 and BT-474, cultured under normoxia or hypoxia was measured by ELISA assay. (C) Addition of anti-TGF-β antibodies was revealed to significantly reduce the effects of exosomes on [3H] thymidine uptake by proliferating splenocytes. Bars represent mean ± standard error of the mean numbers of transmigrated cells from three independent experiments. *P<0.05. TGF-β, transforming growth factor-β; IL-10, interleukin-10; TdR, radioactive thymidine.