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Phage ELISA to identify antigen binding clones. After three rounds of biopanning, selected phage was rescued and antigen-binding clones were detected by phage ELISA. In this experiment 5S hybridoma supernatant and helper phage M13 KO7 were used as positive (+) and negative (–) control respectively. Clone 1 was used for soluble expression of the chimeric Fab.
