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. 2005 Dec 28;11(48):7569–7578. doi: 10.3748/wjg.v11.i48.7569

Figure 4.

Figure 4

Binding properties of the anti-HBs chimeric Fab. 1 Solid phase ELISA was performed with different dilutions of the soluble chimeric Fab. The result of the competitive ELISA between the chimeric Fab and the mouse monoclonal 5S is shown in Figure (B). Different amounts of the chimeric Fab were allowed to compete with 1:80 dilution of the 5S-hybridoma culture supernatant for binding to HBsAg. Bound mouse monoclonal was detected with anti-mouse HRP and the % inhibition of binding was calculated.