Table 1.

The primers used to localize the Vb7 specific amplification in D2139

No.	Primers name	Primer sequences (5’–3’)	Wheat bin map	Restriction enzyme	Length of Vb7 bands
1	TNAC1776	F: ATCATCCTGCTGCTACTGTGC	7AS2-0.73–0.83	–
		R: CCTTCTCAGCTTAGCGATGTG	4AL2-0.75–0.80
			7DS4-0.73–1.00
2	TNAC1782	F: TCACTGAACAGCCTAGACATGG	7AS2-0.73–0.83	HaeIII	690 bp
		R: ATTCGCAGACCGCATCTATC	7BS2-0.27–1.00
			7DS4-0.73–1.00
3	TNAC1803	F: TGCGACCAGTCTCTTTGAAAT	C-7AL1-0.39	HaeIII	800 bp*
		R: GTCGGAGCCTGGATCTCTAGT	7BL2-0.38–0.63
			7DL5-0.30–0.61
4	TNAC1806	F: ATTCCTCGTGAATTGCTGGAT	7AS8-0.45–0.59	TaqI	350 bp*
		R: TCTGCAGTTAGGGACTTGAAA	7BS2-0.27–1.00
			7DS2-0.61–0.73
5	TNAC1811	F: CTGCTCAACGAGTTCATCGAC	7AL1-0.39–0.63	TaqI	740 bp
		R: TTGGAGTGGACGTTGCATT	7BL2-0.38–0.63
			7DL5-0.30–0.61
6	TNAC1812	F: ACTTCGCTTGGTCTCCTCAAT	7AL5-0.63–0.71	TaqI	860 bp
		R: GAGAAGTGTGCCAATTCCAAA	7BL7-0.63–0.78
			7DL5-0.30–0.61
7	TNAC1815	F: AGCAGACATCAGCAAGTTTGAG	7AL1-0.39–0.63	TaqI	600 bp*
		R: ACTGACAAGCCCATGATTGAC	7BL2-0.38–0.63
			7DL5-0.30–0.61
8	TNAC1822	F: CCCTCCGTCCGTGCAAAT	7AL5-0.63–0.71	TaqI	730 bp*
		R: GGCTGATGATGGAGACGTG	7BL2-0.38–0.63
			7DL2-0.61–0.82
9	TNAC1867	F: GCCTTTCCTTTGGTAGTCTGG	C-7AL1-0.39	–	840 bp
		R: CGATCCAAATGATCCTGAAGA	7BL2-0.38–0.63
			7DL1-0.14–0.30
10	TNAC1903	F: TCGCTTCTTCTGCTTGTTCTT	C-7AL1–0.39	TaqI	920 bp*
		R: CTGCTACTAGGCCACCCAAA	C-7BL2–0.38
			7DL1-0.14–0.30
11	TNAC1926	F: CGTCAGCTACAGCGACATCTA	C-7AS8–0.45	TaqI	700 bp*
		R: AACTTGAGCAGCGTGGTGTT	7BS2-0.27–1.00
			7DS3-0.15–0.36
12	TNAC1943	F: GCTGCTATGGTCCACGAATTA	7AS5-0.59–0.73	HaeIII	600 bp*
		R: AGAGTATCGTATCCGGGCAAT	7BS2-0.27–1.00
			7DS4-0.73–1.00
13	TNAC1957	F: TCAACATTTGCAGGATTGTCA	7AL21-0.74–0.86	–	730 bp
		R: TTTCACAGGAACCTCTGCATC	7BL10-0.78–0.84
			7DL2-0.61–0.82

The primers and the location in wheat bin map were referred to Ishikawa et al. [18]. The star indicated the Vb7 specific bands were polymorphic to 7V band