Fig. 1.

The NuRD co-repressor interacts with the human and murine L1 promoters in vivo. MCF7 breast cancer cells and early passage MEF cells were grown to 90 % confluence, crosslinked with DTBP and then treated with 1 % formaldehyde. Solubilized chromatin was immunoprecipitated using selected antibodies and amplified for the known MeCP1 target promoter Snail a, the human L1 promoter b, the murine L1Md-A type promoter c and the human IL-4 intron 2 transcriptional enhancer (d). Panel (a) shows two replicates for each for Mi2-β, MTA2, RbAp46/48, MBD3, MTA3 and IgG. Panel (c) shows the average of three separate chromatin immunoprecipitation assays for NuRD constituent proteins in WT and RB null MEFs (TKO). PCR products were separated in a 1 % agarose gel and visualized using a KODAK imager U.V. station. The results of three different experiments established the interaction of NuRD constituent proteins with the L1 promoter and implicated RB proteins in this process. Statistical differences for non-parametric data were evaluated using the Mann–Whitney test