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. 2016 Jan 25;9:4. doi: 10.1186/s13045-016-0234-9

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© Ventura Ferreira et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Hematopoietic stem cell characterization in β-TCP scaffolds in vitro. C-kit⁺ cells were subjected to the different β-TCP/matrix hybrids over 14 days in vitro. a Percentage of DAPI⁻-viable cells after 14 days of c-kit⁺ cell culture with and without collagen I/III/Matrigel® on β-TCP scaffolds in the presence of MSC support. b Expansion/decrease of lin⁻Sca1⁺c-kit⁻ stromal cells normalized to day 4 of the culture. c Expansion/decrease of lin⁻ normalized to day 4 of the culture. Mean ± SD of three independent experiments. d Colony-forming potential of cells isolated from 500- and 800-μm β-TCP scaffolds with and without collagen I/III/Matrigel® in the presence of MSC support at day 4 and day 14. Results are mean ± SD of three independent experiments. CFU colony-forming unit, GEMM granulocyte-erythrocyte-macrophage-megakaryocyte, GM granulocyte-monocyte. without

Inline graphic — Hematopoietic stem cell characterization in β-TCP scaffolds in vitro. C-kit⁺ cells were subjected to the different β-TCP/matrix hybrids over 14 days in vitro. a Percentage of DAPI⁻-viable cells after 14 days of c-kit⁺ cell culture with and without collagen I/III/Matrigel® on β-TCP scaffolds in the presence of MSC support. b Expansion/decrease of lin⁻Sca1⁺c-kit⁻ stromal cells normalized to day 4 of the culture. c Expansion/decrease of lin⁻ normalized to day 4 of the culture. Mean ± SD of three independent experiments. d Colony-forming potential of cells isolated from 500- and 800-μm β-TCP scaffolds with and without collagen I/III/Matrigel® in the presence of MSC support at day 4 and day 14. Results are mean ± SD of three independent experiments. CFU colony-forming unit, GEMM granulocyte-erythrocyte-macrophage-megakaryocyte, GM granulocyte-monocyte. without