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. 2016 Jan 25;9:4. doi: 10.1186/s13045-016-0234-9

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© Ventura Ferreira et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of trilineage differentiation of c-kit⁺ HSPCs on β-TCP scaffolds in vitro. a Differentiation potential of c-kit⁺ cells cultured for 4 and 14 days on β-TCP scaffolds with and without collagen I/III gel/Matrigel® in the presence of MSC support: granulocytes (Gr1⁺CD11b⁺), monocytes (Gr1⁻CD11b), erythroid precursor cells (Gr1⁻CD11b⁻CD3⁻CD19⁻), and T cells (Gr1⁻CD11b⁻CD3⁺). Percentage of viable cells is shown as mean ± SD of three independent experiments. b SEM of c-kit⁺ cells cultured on 500-μm β-TCP/Matrigel® scaffolds. Very left column highlights cell spreading and detailed cell morphology of day 4 and day 14 cultures. C-kit⁺-derived cells (red arrows) are in close contact to MSCs (white arrows). Cell extensions at day 4 in detail (green arrow). View from the interior of the β-TCP macropores at day 14 (asterisk)