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. 2016 Jan 25;9:4. doi: 10.1186/s13045-016-0234-9

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© Ventura Ferreira et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — a SEM imaging of the explanted scaffolds 4 weeks after transplantation. White arrows: erythrocytes; black arrows: T cells; red arrows: thrombocytes; gray arrow: neutrophils/granulocytes; asterisk: β-TCP. b-c Gating strategy for the flow cytometry: Hematopoietic stem (LSK) and progenitor cells (LK) were identified by being negative for lineage markers and by the expression of Sca1 and c-kit. Lineages were identified by the following stainings: granulocytes (Gr1⁺CD11b⁺), monocytes (Gr1⁻CD11b⁻), erythroid precursor cells (Gr1⁻CD11b⁻CD3⁻CD19⁻), T cells (Gr1⁻CD11b⁻CD3⁺), B cells (Gr1⁻CD11b⁻CD19⁺). d Flow cytometry analysis of the explanted scaffolds 4 weeks after transplantations. Surface marker expression is shown as a percentage of the viable cell population; mean ± SD of three independent experiments