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HPLC profiles for the conversions of linoleic acid to putative hydroxy fatty acids by crude enzyme extracts from P. chrysogenum (PC), P. digitatum (PD), P. oxalicum (PO), and P. marneffei (PM). Compounds used as standards, including 5S,8R-DiHODE, 7S,8S-DiHODE, 8R,11S-DiHODE, 8R-HODE, 10R-HODE, and linoleic acid, are shown. Crude P. chrysogenum (PC_1) enzyme extract produced a putative 8R,11S-DiHODE. The peak at about 7.7 min represents EPPS buffer.
