Fig. 3.

Macrophage cholesterol efflux capacity, ABCA1 efflux capacity, and HDL size in the different strains of mice. A: HDL size was measured by calibrated IMA in HDL isolated from serum by ultracentrifugation. Serum HDL was obtained by polyethylene glycol precipitation of APOB-containing lipoproteins from plasma-derived serum. Macrophage cholesterol efflux capacity (cAMP-stimulated J774 macrophages) (B) and ABCA1-specific cholesterol efflux (mifepristone-stimulated BHK cells minus BHK cells incubated in medium alone) (C) were measured after a 4 h incubation with serum HDL (2.8% v/v) as described in the Methods.