TABLE 1.
Calculations for cholesterol biosynthesis and uptake rates for mouse retina and brain
| Line # | Experimental Data/Calculated Values | Serum | Retina | Brain |
| Mice exposure to dietary [2H7]cholesterol | ||||
| 1 | [2H7]cholesterol, % | 36.5 ± 1.1 | 1.3 ± 0.1 | 0.09 ± 0.01 |
| 2 | Cholesterol uptake per week, % | ND | 3.6 | 0.2 |
| Mice exposure to deuterated water | ||||
| 3 | 2H cholesterol enrichment originating from uptake, % | ND | 0.29 | 0.02 |
| 4 | Total 2H cholesterol enrichment, % | 8.1 ± 1.0 | 9.7 ± 0.9 | 6.3 ± 0.7 |
| 5 | Cholesterol biosynthesis per week, % | ND | 9.4 | 6.3 |
| Summary data | ||||
| 6 | Cholesterol content, mg/g wet tissue | ND | 1.13 ± 0.08 | 13 ± 1 |
| 7 | Absolute rate of cholesterol input, μg/g wet tissue • day | ND | 21 | 121 |
| 8 | Local biosynthesis | ND | 15 | 117 |
| 9 | Uptake from blood | ND | 6 | 4 |
| 10 | Relative rate of cholesterol input, %/week | ND | 13.0 (100%) | 6.5 (100%) |
| 11 | Local biosynthesis | ND | 9.4 (72%) | 6.3 (97%) |
| 12 | Uptake from blood | ND | 3.6 (28%) | 0.2 (3%) |
ND, not determined. Lines in italics represent experimental data; lines in bold indicate the final calculated values, which were determined by using the means of experimental data; ± values represent SD. Animal statistics are indicated in the Materials and Methods.