TABLE I. Results of microscopic examination, TaqMan polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism (RFLP) 18S rDNA, and by gp60 PCR/sequencing of faecal samples from hospitals of the city of Rio de Janeiro, Brazil, forCryptosporidium characterisation.
Sample | Microscopy | Real-time PCR | 18S rDNA/RFLP | gp60/sequencing |
---|---|---|---|---|
Pc1 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc2 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc6 | Negative | C. hominis | No amplification | No amplification |
Pc7 | Negative | C. hominis | C. hominis | IbA10G2 |
Pc8 | Negative | Cryptosporidium spp | No amplification | No amplification |
Pc13 | Negative | Cryptosporidium spp | No amplification | No amplification |
Pc16 | Cryptosporidium spp | Cryptosporidium spp | No amplification | No amplification |
Pc17 | Cryptosporidium spp | No amplification | No amplification | No amplification |
Pc20 | Cryptosporidium spp | Cryptosporidium spp | C. hominis | IbA10G2 |
Pc27 | Negative | C. hominis | C. hominis | IbA10G2 |
Pc28 | Negative | C. parvum | No amplification | No amplification |
Pc44 | Cryptosporidium spp | No amplification | No amplification | No amplification |
Pc46 | Cryptosporidium spp | No amplification | No amplification | No amplification |
Pc52 | Negative | C. hominis | C. hominis | IbA10G2 |
Pc78 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc79 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc80 | Cryptosporidium spp | C. hominis | C. hominis | Cryptosporidium |
Pc81 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc82 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc83 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc84 | Cryptosporidium spp | C. hominis | C. hominis | IbA10G2 |
Pc85 | Cryptosporidium spp | C. hominis | C. hominis | Cryptosporidium |
Pc86 | Negative | C. parvum | No amplification | IbA10G2 |
Pc87 | Negative | C. parvum | No amplification | Cryptosporidium |
Pc88 | Cryptosporidium spp | C. parvum/C. hominis | C. parvum/C. hominis | Cryptosporidium |
Pc89 | Cryptosporidium spp | C. parvum | C. parvum | IIcA5G3 |